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What You Need to Know About Electrophysiology

What You Need to Know About Electrophysiology

What Is Electrophysiology?

This test is performed on one’s heart by accessing the heart’s electrical activity to diagnose unusual heartbeats and more. The test is performed by inserting catheters and wired electrodes (to measure electrical activity) into one’s blood vessels. An electrophysiologist is someone who specifically deals with the electrical part of this process. The number of people suffering from cardiac diseases has increased in recent times in Sydney. Electrophysiology is a critical test globally, but it has been progressing a lot in Sydney. It’s important to choose the best electrophysiologists in Sydney for your condition.

Advantages and Disadvantages of Electrophysiology

This test can:

– Sometimes predict the risk of a sudden cardiac arrest/heart attack.

– Help figure out the cause of the disturbances in one’s heart’s rhythm (arrhythmias). 

Although this test has quite a lot of benefits, it also has a few drawbacks.

– It provides no anatomic information about the PN (promissory note)

– As the definition suggests, it is painful and uncomfortable for the patient.

– It is challenging to get the needle inside the thick muscles in one’s body.

However, the advantages of this technique outweigh the disadvantages, due to which any electrophysiologist in Sydney prefers this test.

Types of Electrophysiological Probes

Single electrode – a single electrode is inserted through an implanted chamber of an animal brain (example).

Multielectrode array – they are a grid of multiple electrodes capable of recording the activity of several neurons on the surface of the brain. The mass and size are too big to be implanted inside the brain, which is why they are implanted on its surface.

Tetrode – it is a type of multielectrode array but with four active electrodes. The only difference is that a tetrode is narrower and can be inserted into deep parts of the brain with ease.

Patch-Clamp Technique

The patch-clamp technique is a kind of technique that is used in laboratories by an electrophysiologist in sydney to study the ionic current in an individual being. In short, it is a laboratory technique to study the current in the living cells of a human being.

This technique helps us understand the channel behaviour of an ion inside the cell of a human being. A patch-clamp technique uses axon instruments like digitisers, amplifiers, software and accessories. First, electrophysiologists prepare the solution and then prepare the cells or the brain slices (which are isolated). After that, they put it in the polyester pipette. After all of this, they set up the perfusion system that is used for data acquisition. Then they patch up the cell to complete the process. And this is how they get there if something is wrong with someone.

There are five types of patch clamping techniques.

  1. Cell patch clamping – A pipette is secured to the cell/plasma membrane to measure the charge through the ion channels in the area of the membrane in cell-attached patch clamping. The cell membrane is still intact. The benefit of attaching only to the outside of the membrane is that the inner cell structure is not disrupted, allowing intracellular mechanisms to function normally.
  1. Whole-cell patch clamping: A whole-cell patch-clamp measures currents flowing through the cell membrane as a whole. It is done the same way as cell-attached patch clamping, but more suction is used to rupture the membrane.
  1. Inside out patch-clamp – The inside out patch-clamp works by affixing a piece of the cell membrane to the glass tube, exposing the cytosolic surface. It allows for access to the surface via the electrolyte solution bath.
  1. Outside out patch-clamp – Outside-out patch clamping, like inside-out patch clamping, begins with a whole-cell technique.
  1. Loose patch-clamp – this is similar to cell clamping, except the seal used is lighter.

What You Need to Know About Electrophysiology

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