What Are The Rules For Collecting Specimens?

Collecting a urine specimen for drug testing is one of the most common methods of drug testing. While most organizations use the U.S. Department of Transportation (DOT) rules for collecting these specimens, there are certain situations that require the collection of a non-DOT urine specimen.

This type of specimen collection is subject to different rules and regulations than DOT collections, so it’s important to understand them in order to ensure compliance and accuracy. In this article, we’ll explore what these rules are and how they differ from DOT collections.

What Does a DOT Urine Collection Involve?

Before we go into the specifics of collecting non-DOT urine specimens, let’s take a look at what the process of DOT urine collection actually involves. Generally, it consists of a trained collector (usually a medical professional) who will observe and supervise the process while taking all necessary precautions to ensure that samples are properly collected, labeled, and handled. This could include collecting specimens in two different containers (one primary and one secondary) as well as sealing the samples properly and labeling them with the necessary identifying information.

Rules for Collecting DOT Urine Specimens

Now that we know what is involved in collecting DOT urine specimens let’s look at some of the rules that must be followed. First, there should always be two collectors present when collecting a sample – one for the primary collection and one for the secondary collection.

This ensures that each container is properly labeled with its own unique identification number. It’s also important to make sure that the collectors don’t cross-contaminate any containers during the collection process.

In addition to having two collectors present during the sample collection process, there are several other safety measures that must be taken in order to maintain an accurate sample. For example, it is required that all containers used for sample collection have tight-fitting lids, as this will help prevent contamination or leakage of the specimen.

The collectors should also wear gloves while handling any part of the specimen collection process to avoid skin contact with any potentially contaminated materials. Finally, any sample that is collected must be stored in a temperature-controlled environment until it can be shipped to a laboratory for further analysis.

What is a Non-DOT Urine Specimen Collection?

NON-DOT Urine Specimen Collector Train-the-Trainer is a type of drug testing training that does not follow the guidelines set by the U.S. Department of Transportation. Non-DOT tests are typically used for pre-employment screening, random drug tests, or follow-up tests after an employee has failed a DOT test. These tests can be conducted either onsite at an employer’s location or at a third-party laboratory or clinic.

What Are the Benefits of Non-DOT Urine Specimen Collection?

There are several advantages to using non-DOT urine specimen collection instead of DOT collections. First, non-DOT specimens do not have to adhere to the same stringent standards as DOT specimens, which means employers can customize their testing protocols based on their needs and budget.

Second, since non-DOT tests are conducted by third parties, employers can avoid any potential conflicts of interest when it comes to drug testing their employees. Third, since non-DOT specimens are typically analyzed at a lab or clinic rather than onsite, employers can get more reliable results without having to invest in additional equipment or training personnel.

What Are the Rules for Collecting Non-DOT Urine Specimens?


When collecting a non-DOT urine specimen, employers must ensure that the sample is stored securely and appropriately. The sample must be kept in a locked container at all times until it is sent off for analysis.


All specimens must be labeled with the date and time they were collected, as well as the name of the collector and any other relevant information, such as the donor’s name or ID number.


The donor must be monitored at all times while providing their sample in order to ensure that no tampering occurs and that the integrity of the sample is maintained throughout the process.

Chain of Custody:

A chain of custody form must be completed when collecting a non-DOT urine specimen in order to document the process from start to finish and provide an audit trail should any questions arise about the validity of the sample later on down the road.

Testing Method:

Employers must also decide which type of testing method they want to use for their non-DOT specimens; this could include immunoassay or gas chromatography/mass spectrometry (GC/MS). Immunoassay tests are usually less expensive but may have higher false positive rates, whereas GC/MS tests are more accurate but tend to be more expensive.


Non-DOT urine samples should be sent off for analysis as soon as possible after they have been collected in order to ensure accuracy, ideally within 24 hours if possible, though this depends on specific circumstances such as the availability of laboratory personnel and so forth. 


Employers must also take steps to ensure that donor privacy is maintained throughout the process; this includes keeping confidential information such as donor identity confidential as well as ensuring that all documents related to drug testing are kept secure and not shared with anyone outside of those involved in conducting or analyzing the test results.


Employers should maintain comprehensive documentation regarding all aspects of their non-dot drug testing program, including who was tested, when they were tested, what type of test was used, where the test was conducted, etc., in order to ensure compliance with applicable laws and regulations as well as protect themselves from potential legal action should any problems arise down the road.


Those involved in collecting non-dot urine specimens should receive appropriate training on how to collect samples properly as well as how to handle them securely during transport and storage prior to analysis by a laboratory or clinic. Training should also include familiarizing personnel with any applicable laws and regulations related to drug testing so they can ensure compliance throughout every step in the process.

Retention Policy:

Finally, employers should establish a retention policy regarding any records related to their drug testing program, including documents such as chain of custody forms and test results; this will help protect them from potential legal issues should any arise down the road due to improper handling or storage of records related to drug testing activities performed by their organization. 


Collecting a non-dot urine specimen requires adherence to certain rules and regulations that differ from those used for DOT collections. Employers must store samples securely; label them correctly; maintain donor privacy; provide appropriate training; document all activities related to their drug testing program; establish a retention policy; and select an appropriate testing method such as immunoassay or GC/MS depending on their needs and budget constraints.

By understanding these rules and taking steps to comply with them, employers can ensure accurate results from their drug testing activities while avoiding potential legal issues down the line due to improper handling or storage of records related to their drug testing program.

What Are The Rules For Collecting Specimens?